High-Performance Sequencing

From microbial genomes through to highly polyploid plants, we provide access to whole-genome sequencing workflows across all our platforms, including bioinformatics support.

Whether it’s re-sequencing a model organism, performing population-scale diversity studies, or assembling a high-quality genome from a new species, we can work with you to identify the approach that best suits your needs, from long-read HiFi sequencing on the PacBio Revio, to ultra-high throughput sequencing on the Illumina NovaSeq X with short reads. 

We support transcriptome sequencing and analysis, from short-read based transcript reconstruction approaches on the NovaSeq X, to full-length RNA sequencing on the PacBio Revio, including the latest Kinnex technology.

We can also help with data analysis for model and non-model organisms to identify the location and structure of genes, quantify gene and transcript expression, and explore differences between experimental conditions.

Community profiling of microbiomes can be done in a number of ways.

We use a short-read, high-throughput metabarcoding workflow which targets amplicons of interest (e.g. 16S, 18S, ITS) for rapid interrogation of mixed communities from human and environmental samples.

If you are interested in the biochemical pathways present in a specific community, we can also offer whole genome shotgun metagenomics using a combination of low-cost LITE 2.0 library preparation and short-read sequencing on the NovaSeq X or we can generate long-read HiFi data for high-quality MAG reconstruction on the PacBio Revio. .

Both approaches are supported by our Core Bioinformatics group who can provide a taxonomic profiling / composition analysis.

Being able to look at the transcriptional dynamics, or genetic variation between single cells is a powerful tool in genomics.

We support complete workflows for single-cell RNA-seq analysis (Smart-seq2) and joint DNA/RNA analysis (G&T-seq) for selected cell types, from provision of cell lysis plates through to library preparation, sequencing and preliminary data analysis.

For bespoke single-cell projects, our single-cell team can assist with cell sorting, 10X Genomics applications and other methods, including single-cell RNA and DNA sequencing on a wide variety of organisms.

Our Core Bioinformatics team is highly experienced in large-scale data analysis and familiar with data from most next-generation sequencing platforms.

We can provide bioinformatics support across a range of analyses, including genome and transcriptome assembly, genome annotation, gene expression analysis, variation detection, microbial community profiling and metagenome / metatranscriptome assembly with functional and taxonomic assignment.

We use open source tools as well as those developed in-house to support data-intensive research at the Earlham Institute.

Bioinformatic analysis is normally offered in conjunction with sequencing services, but we are also happy to discuss how we can support analysis of externally generated data.