Introduction to Flow Cytometry

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The workshop will improve theoretical knowledge in flow cytometry including:

• Understanding of fluorescence and fluorochromes: principles of fluorescence, excitation and emission spectra, different classes of dyes used in fluorescence technologies. 
• Understand how a flow cytometer works: The fluidics, optics and electronics systems found on all flow cytometers; How to use this knowledge to help troubleshoot;
• Considerations of conventional and full spectrum cytometry
• Gain knowledge in compensation and data spread: How fluorescence spillover can be corrected; the right controls to bring to the cytometer to ensure successful compensation or unmixing
• Best practice in experimental design and sample preparation: How to use knowledge of fluorescence and the cytometer to design successful multicolour experiments;
• Which factors need to be considered in running an experiment (sample preparation, staining, controls, quality control)
• The principles of droplet sorting using the FACS Melody as an example including Quality Control and correctly setting drop delay
• Applications of cell sorting
• How to select the cells that you are interested in
• Learn the principles of gating and deriving the most meaningful statistic
• How to present flow data to an audience
• Learn of new technologies: Including BD S8

Prerequisites:

No previous experience required. Preference will be given to those in the early planning stages of their projects.

Target Audience:

Ideal for researchers starting out in flow cytometry and in the planning stages of an experiment. An opportunity to expand theoretical knowledge for those more experienced researchers.