Articles | Earlham Institute

Learning Expert

FEATURE | 10 min READ

How to design and use MoClo in budding yeast - part 2

Constructing a custom CRISPR/Cas9 plasmid using Golden Gate assemblies for budding yeast transformations.

Learning Expert

FEATURE | 8 min READ

How to design and use MoClo in budding yeast - part 3

Transforming budding yeast with Golden Gate assembled healing fragments and custom CRISPR/Cas9 plasmid.

Learning Expert

FEATURE | 10 min READ

How to design and use MoClo in budding yeast - part 1

Designing and building your integration plasmid for Golden Gate assemblies and transformation into budding yeast.

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