Event Scientific training

miRNA Workshop 2016

This workshop is aimed at early career researchers looking to analyse small RNA data produced from High Throughput Sequencing experiments on both plant and animal samples

Start date: 27 June 2016

End date: 28 June 2016

Time: 08h30 - 16h30

Venue: Earlham Institute

Organiser: Emily Angiolini

Enquiries:

training@earlham.ac.uk

Registration deadline: 13 May 2016

Cost: £50.00

About the event

What is the course about?
Analysis of Small RNA data produced from High Throughput Sequencing experiments on both plant and animal samples.

What will I Learn?
Initial preparation of small RNA data including quality control and normalisation of samples, searching for miRNA sequences and their respective targets, detection of differentially expressed sequences and building sRNA target interaction network diagrams. Finally, discussions will take place on experiment design and how this can impact overall analysis strategies.

Course Prerequisites
Biological or Computer science (computational biology) degree and either completed or in process of completion of post graduate study in a related discipline.

Target Audience
Early career bioinformatics post doc, bioinformatics/computational biology PhD students, biology post doc/PhD students with an interest in bioinformatic analysis.

Download course materials

All of the training resources used in this course are provided for free.

Organisers

Programme

Day 1 - 27 June 2016

08:30 - 09:00 Registration and coffee

09:00 - 10:30 Welcome, introductions, trainee presentations

10:30 - 10:45 Coffee break

10:45 - 12:15 Raw data processing - quality control - normalisation - annotation

12:15 - 13:00 Lunch

13:00 - 14:30 Overview of miRNAs, how to analyse miRNA prediction data, how to spot false positives

14:30 - 16:00 Using miRNA prediction tools - setup and use of miRDeep and miRCat

16:00 - 16:15 Coffee break

16:15 - 17:45 Differential expression of sRNA data - setup and use of the workbench differential expression tool

Day 2 - 28 June 2016

09:00 - 10:30 Morning Coffee

10:30 - 10:45 Coffee break

10:45 - 12:15 Improvements to miRNA target prediction - setup and use of target prediction software

12:15 - 13:00 Lunch

13:00 - 14:30 sRNA interaction networks overview - using network generation software

14:30 - 14:45 Coffee break

14:45 - 16:15 Q&A - experimental design

16:15 - 16:30 Feedback and wrap up

Further information

FREE one day meeting focused on Small RNAs to be held at UEA

One-day national meeting on the study of small RNAs in all areas of molecular and cell biology. Which will bring together early and experienced scientists from all areas of the field, from plant biology to cancer biology, to discuss their current experiments and ideas. The plenary speaker for the meeting is Professor Sir David Baulcombe.

Registration is FREE and open to PhD students, postdocs and principle investigators. Registration includes free parking at the event, food and drink breaks and a lunch networking opportunity. During registration, please indicate if you would like to present a talk or poster by sending a 300 word abstract. Presenting a talk or poster is optional and registration can be completed without them. Please also indicate any dietary requirements or food allergies, or requirement for disability access.